TA Cloning Kit-20 test

توضیحات

محتویات کیت:
1 – Master mix 2x 500ul 125ul
2 – Sterile DW 900ul 225ul
3 – pTG-19T vector (25ng/μl) 40ul 10ul
4 – 10X Buffer Ligase 20ul 5ul
5 – T4 DNA Ligase (200u/μl) 20ul 5ul
6 – Control insert (12.5ng/μl) 20ul 10ul
7 – Ampicillin (50 mg/ml) 1000ul 500ul
8 – IPTG 100mM 1600ul 400ul
9 – X-Gal (20 mg/ml) 2000ul 500ul
10 – LB medium 60ml 15ml
11 – Buffer S 14.4ml 3.6ml
12 – M13 Forward Primer 20ul 5ul
13 – M13 Reverse Primer 20ul 5u